Structural and Functional Characterization of 4-Hydroxyphenylacetate 3-Hydroxylase from Escherichia coli

The hydroxylation of phenols into polyphenols, which are valuable chemicals and pharmaceutical products, is a challenging reaction. The search for green synthetic processes has led to considering microorganisms and pure hydroxylases as catalysts for phenol hydroxylation. Herein, we report the structural and functional characterization of the flavin adenine dinucleotide (FAD)-dependent 4-hydroxyphenylacetate 3-monooxygenase from Escherichia coli, named HpaB. It is shown that this enzyme enjoys a relatively broad substrate specificity, which allows the conversion of a number of non-natural phenolic compounds, such as tyrosol, hydroxymandelic acid, coumaric acid, hydroxybenzoic acid and its methyl ester, and phenol, into the corresponding catechols. The reaction can be performed by using a simple chemical assay based on formate as the electron donor and the organometallic complex [Rh(bpy)Cp*(H₂O)]²⁺ (Cp*: 1,2,3,4,5-pentamethylcyclopentadiene, bpy: 2,2′-bipyridyl) as the catalyst for FAD reduction. The availability of a crystal structure of HpaB in complex with FAD at 1.8 Å resolution opens up the possibility of the rational tuning of the substrate specificity and activity of this interesting class of phenol hydroxylases.     

Formaldehyde as a Chemical Defence Agent of Fruiting Bodies of Mycena rosea and its Role in the Generation of the Alkaloid Mycenarubin C

Mycenarubin C, a previously unknown red pyrroloquinoline alkaloid, was isolated from fruiting bodies of the mushroom Mycena rosea and its structure was elucidated mainly by NMR spectroscopy and mass spectrometry. Unlike mycenarubin A, the major pyrroloquinoline alkaloid in fruiting bodies of M. rosea, mycenarubin C, contains an eight-membered ring with an additional C₁ unit that is hitherto unprecedented for pyrroloquinoline alkaloids known in nature. Incubation of mycenarubin A with an excess of formaldehyde revealed that mycenarubin C was generated nearly quantitatively from mycenarubin A. An investigation into the formaldehyde content of fresh fruiting bodies of M. rosea showed the presence of considerable amounts of formaldehyde, with values of 5 μg per gram of fresh weight in fresh fruiting bodies. Although mycenarubin C did not show bioactivity against selected bacteria and fungi, formaldehyde inhibits the growth of the mycoparasite Spinellus fusiger at concentrations present in fruiting bodies of M. rosea. Therefore, formaldehyde might play an ecological role in the chemical defence of M. rosea against S. fusiger. In turn, S. fusiger produces gallic acid—presumably to detoxify formaldehyde by reaction of this aldehyde with amino acids and gallic acid to Mannich adducts.     

生物预处理技术在天然产物提取中的应用研究进展

生物预处理技术运用于天然产物提取是借助微生物或生物酶的作用，在适当条件下对天然资源进行处理，破坏植物原料的细胞壁，促进天然产物释放到提取介质中，以提高分离效率，有助于保证天然产物活性。生物预处理技术按形式可以分为微生物发酵技术和生物酶处理技术。针对2种生物预处理技术的作用机制和应用进行综述，旨在为天然产物开发利用提供参考。     

季戊四醇锌与月桂酸锌并用对天然橡胶硫化特性的影响

采用季戊四醇锌与月桂酸锌并用作为硫化活性剂,考察其对天然橡胶硫化特性及物理机械性能的影响。结果表明,季戊四醇锌具有较高的硫化活性,可使胶料的硫化速率加快,硫化活化能下降,硫化活性高于月桂酸锌,但抗硫化返原性能较差,胶料的物理机械性能较低,耐磨性较差。将季戊四醇锌与月桂酸锌并用可产生硫化协同效应,明显提高胶料的表观交联密度,改善抗硫化返原性能,同时还能降低门尼黏度、提高填料分散性并降低生热和滚动阻力。加入6.70份季戊四醇锌并用5.56份月桂酸锌的天然橡胶硫化胶,其综合性能优于添加5.00份氧化锌的硫化胶,并且含锌质量分数减少48%。     

铸造式和焊接式主轴箱体的动态特性对比分析

利用有限元法和试验模态法对立式加工中心的铸造式和焊接式两种结构的主轴箱体进行了模态分析,分别获得了其前六阶固有频率和振型。通过对比两种方法获得的结果,验证了有限元分析模型的有效性。研究表明,两种主轴箱体的阻尼比接近,但焊接式主轴箱体的各阶固有频率均要高于铸造式主轴箱体的各阶固有频率。并且铸造式主轴箱体的固有频率更接近于机床主轴的常用工作频率,焊接式主轴箱体的则远离此频率。因此对于常用主轴转速而言,焊接式主轴箱体的动态性能要优于铸造式主轴箱体的动态性能。     

天然染料敏化太阳能电池性能模拟研究

基于电子传输的扩散理论建立了染料敏化太阳能电池（DSSC）的连续性方程,并在扩散方程的基础上,将单一天然染料及混合天然染料的吸收光谱参数引入连续性方程中,对模拟得到的天然染料的伏安特性曲线进行分析与评价,得到了高效率的天然染料及最优天然染料组合。本研究对天然染料敏化太阳能电池的应用与技术发展具有一定的指导意义。     

Marangoni convective nanofluid flow over an electromagnetic actuator in the presence of first‐order chemical reaction

The present study aims to investigate Marangoni‐forced convective nanofluid flow over an electromagnetic actuator (Riga plate). A first‐order homogeneous chemical reaction is considered. The thermocapillary and solutocapillary Marangoni effect developed by the surface tension is considered as a driving force for the nanofluid. In addition, Grinberg‐term is accounted to involve the impact of Lorentz force impinged by the actuator in the model. A set of nonlinear ordinary differential equations is obtained via suitable transformations for a nonsimilar analysis. Series solutions are achieved through homotopy to discuss the behavior of the velocity field, thermal distribution, and concentration of the nanoparticles graphically. The variation in Nusselt and Sherwood numbers is discussed. The outcomes declared that the flow parallel to the surface of the plate is assisted by the Lorentz forces generated by electromagnetic bars of the actuator resulting in an enhancement in the fluid motion. Furthermore, the stronger Marangoni effect resulted in the declining trend of the temperature profile. The concentration of nanoparticles near the surface reduced intensive chemical reaction inside the nanofluid.     

Nonlinear convection in an elasticoviscous fluid‐saturated anisotropic porous layer using a local thermal nonequilibrium model

By adopting a perturbation method and a local thermal nonequilibrium model, nonlinear thermal convection in an anisotropic porous layer saturated by an elasticoviscous fluid is investigated. An elasticoviscous fluid is modeled by a modified Darcy‐Oldroyd‐B model, and the fluid and solid phase temperatures are represented using a two‐field model for the heat transport equation. Anisotropy in permeability and fluid and solid thermal conductivities are considered. A cubic Landau equation is derived separately to study the stability of bifurcating solution of both stationary and oscillatory convection, and the results of linear instability theory are delineated. The boundary between stationary and oscillatory convection is demarcated by identifying codimension‐two points in the viscoelastic parameters plane. It is found that the subcritical instability is not possible, and the linear instability analysis itself completely captures the behavior of the onset of convection. Heat transfer is obtained in terms of Nusselt number, and the effect of governing parameters on the same is discussed. The results of the Maxwell fluid are obtained as a particular case from the present study.     

International Trade

Values of net oil imports(-)/exports for Austria, Belgium, Denmark, Finland, France, Germany, Italy, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, the United Kingdom, Japan, Canada, the United States and Australia. Updated on a monthly basis. Number of imports for Belgium, France, Germany, Greece, Republic of Ireland, Italy, Netherlands, Portugal, Spain, Sweden, the United Kingdom, and Other EU-15 (EU-15), Norway, Poland, Switzerland, Turkey, and Other Europe (OECD Europe), Canada, Chile, Mexico, and the United States (OECD Western Hemisphere), and Australia, Japan, Republic of Korea, New Zealand, (OECD Asia-Pacific), and Total OECED. Current data for Austria, Belgium, Finland, France, Germany, Greece, Italy, Netherlands, Portugal, Spain, Sweden, the United Kingdom, Other EU-15 (EU-15), Czech Republic, Hungary, Poland, Slovakia, Turkey, and Other Europe (OECD Europe), Canada, Mexico, and the United States (OECD Western Hemisphere), Australia, Japan, Republic of Korea, and New Zealand (OECD Asia-Pacific). Updated on a monthly basis. Current data for principal importers of natural gas and the amount in which they import from United States, Republic of Korea, United Kingdom, France, Japan, Italy, Germany and Spain (Table 19.1). Current data for principal exporters of natural gas and the amount they export to Canada, Trinidad and Tobago, the United States, Netherlands, Norway, Russia, Turkmenistan, Qatar, Algeria, Nigeria, Indonesia, Malaysia and Australia (Table 19.2). Updated on a monthly basis.     

新型装配式冰场受力性能试验与模拟分析

为在2022年冬奥会期间将国家游泳中心泳池场地改造为冰壶场地,结合临时场地的新型制冰方式,提出了一种全装配式的冰壶场地及支承结构体系的构造作法.对此装配式冰场进行了现场试验测试和有限元受力性能分析,研究了装配式冰场的自振频率和振型特点,分析了冰场在使用荷载作用下的整体变形和各组件的应力水平,探讨了极端荷载作用下冰面受力、变形及开裂等关键问题.研究表明,所提装配式冰场方案具有较大刚度和承载力,能够满足冬奥会冰壶比赛对冰面的要求；此类冰场宜使用轻型浇冰车作业,大型浇冰车轮压引起的弯矩和剪力作用下冰面存在开裂的可能.